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Flow Cytometry. Nuclear and nonnuclear incorporation of 5-bromo-2'-deoxyuridine in live cells. Exponentially growing promyelocytic leukemia HL60 cells.
Note: You clicked on an external link, which has been disabled in order to keep your shopping session open. Connect Your Lab Anmelden Eilbestellung. Search Thermo Fisher Scientific. Search All. Learn more. Beschreibung Cells can naturally incorporate the thymidine analog BrdU into their cells during cell division, making this nucleoside analog an excellent marker of by cell cycle and proliferation.
Analysis of incorporated BrdU can be with an anti-BrdU antibody. For Research Use Only. Not for use in diagnostic procedures. Detection of cell proliferation by BrdU incorporation. B for 30 minutes before fixation. Incorporated BrdU was detected with mouse monoclonal anti-bromodeoxyuridine antibody Cat. A and diaminobenzidine DAB staining. Both nuclear and non-nuclear presumably mitochondrial incorporation of BrdU is clearly visible in the resulting image.
Exponentially growing human promyelocytic leukemia Cat. C in the presence of exogenous terminal deoxynucleotidyl transferase resulted in incorporation of the fluorophore into DNA strand breaks, thereby labeling the S-phase cells. The DNA was counterstained with the red-fluorescent nucleic acid stain propidium iodide Cat. In addition, the cells were labeled with red-fluorescent propidium iodide Cat. Increased BrdU incorporation is indicative of actively proliferating cells.
The sample was mounted with the reagents in the Prolong Antifade Kit P Thermo Fisher Scientific Inc.
BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Molecular Probes™ BrdU (5-Bromo-2'-Desoxyuridin)