During the last five decades, as a result of an interaction between natural product chemistry, synthetic organic chemistry, molecular biology and spectroscopy, scientists reached an extraordinary level of comprehension about the natural processes by which living organisms build up complex molecules. In this context, 13 C nuclear magnetic resonance spectroscopy, allied with isotopic labeling, played a determinant role. Nowadays, the widespread use of modern NMR techniques allows an even more detailed picture of the biochemical steps by accurate manipulation of the atomic nuclei. This article focuses on the development of such techniques and their impact on biosynthetic studies. Keywords: 13 C NMR; isotopic labeling; biosynthetic routes.
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During the last five decades, as a result of an interaction between natural product chemistry, synthetic organic chemistry, molecular biology and spectroscopy, scientists reached an extraordinary level of comprehension about the natural processes by which living organisms build up complex molecules.
In this context, 13 C nuclear magnetic resonance spectroscopy, allied with isotopic labeling, played a determinant role. Nowadays, the widespread use of modern NMR techniques allows an even more detailed picture of the biochemical steps by accurate manipulation of the atomic nuclei.
This article focuses on the development of such techniques and their impact on biosynthetic studies. Keywords: 13 C NMR; isotopic labeling; biosynthetic routes. Ver, por ex.
Recentemente, foi caracterizada a estrutura cristalina da primeira diels-alderase natural Desaty, D. Tanabe, M. McInnes, A. Simpson, T. Binder, M. Macedo Jr, F. Bax, A. Wang, C. Buddrus, J. Bringmann, G. Lee, M. Houdai, T. Ahmed, A. Staunton, J. Burg, R. Agents Chemother. Cane, D. Duttton, C. Dutton, C. Pohnert, G. Less, S. Williamson, R. Orjala, J.
Hinse, C. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Services on Demand Journal. Secretaria Executiva Av. How to cite this article.
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Nuclear Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy NMR is an information rich, non-destructive analytical technique. It provides detailed information about molecular structure, dynamic processes and allows the direct observation of chemical reactions. It is also a primary quantitative method allowing the determination of concentration of molecules even in complex mixtures. In addition to the well-known observation of hydrogen, carbon, fluorine and phosphorous it can be used on a large number of other elements. NMR is an indispensable tool in chemistry.
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